2008-10-22_REPORT - C1981008 (3)

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• Table 1 - Colorado Chronic Test Conditions Parameter Ceriodaphnia dubia Fathead Minnow Test Type Temperature Photoperiod Test Chamber Size Test Sample Volume Renewal Period Age of Organisms Number per Chamber Chambers per Concentration Feeding Regime per Chamber Test Duration Static renewal 25 +/- 10C 16 hr light/8 hr dark 30 mL 20 mL 24 hrs < 24 hrs 1 10 0.1 ml Algae/YTC per day Until 60% of control organisms have 3 broods or 8 days Static renewal 25 +/- 10C 16 hr light/8 hr dark 600 mL 300 mL 24 hrs < 24 hrs 10 4 0.15 ml Brine shrimp 2x/day 7 days Table 2 - Methods and Equipment Characteristic Method/Equipment EPA Method Alkalinity Titrimetric EPA 310.1 Ammonia CHEMetrics Colorimetery K-1510 Chlorine, Total Residual CHEMetrics Colorimetery K-2504 Conductivity Cole-Parmer Model 1500 Cond. Meter EPA 120.1 Dissolved Oxygen YSI Model 59 DO Meter EPA 360.1 Hardness Titrimetric EPA 310.1 & 130.2 PH Fisher Accumet Model 910 pH Meter EPA 150.1 Temperature NIST5 calibrated thermometer Analytical Balance Mettler AE 240 References Methods for Chemical Analysis of Water and Waste, Environmental Protection Agency, Environmental Monitoring Systems Laboratory - Cincinnati (EMSL-CI), EPA-600/4-79-020, Revised March 1983 Colorado Department of Health 1998 Guidelines for Conducting Whole Effluent Toxicity Tests Water Quality Control Division, Denver, Colorado 80246-1530. US EPA, October 2002, Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, Fourth Edition, U.S. Environmental Protection Agency, • Office of Water, Washington, D.C. EPA-821-R-02-013 5 National Institute of Standards and Technology 5