2008-10-22_REPORT - C1981008 ‎(3)‎

• Permit No: C1981008
• IBM Index Class Name: REPORT
• Doc Date: 10/22/2008
• Doc Name: 2008 AHR Appendix C Part 3
• From: Western Fuels-Colorado
• To: DRMS
• Annual Report Year: 2008
• Permit Index Doc Type: Hydrology Report
• Email Name: MLT
• Media Type: D
• Archive: No

•
Table of Contents
Abstract Table 2 - Methods and Equipment
Methods References
Results Discharge Monitoring Report Form
Narrative & Discussion Reference Toxicity Data Chart(s)
Table 1 - Colo. Test Conditions Supplemental Forms'
Abstract
On September 15, 2008, a two species acute bioassay was conducted with effluent from the
Western Fuels New Horizon Mine, outfali 011. The purpose of the bioassay was to determine if the
facility complies with the acute WET portion of the Colorado State Permit System Authorization to
Discharge. (Reference SGS Sample ID: 411-080-BIOA-069)
. According to the Western Fuels New Horizon Mine discharge permit (CO-0000213), an acute WET test
is failed whenever the LC60, which represents an estimate of the effluent concentration which is lethal to
50% of the test organisms in the time period prescribed by the test, is found to be less than or equal to
100% effluent. Results of the initial testing indicated the sample was acutely toxic to fathead
minnow survival but not to Ceriodaphnia dubia survival.
To determine if the facility complies with the "Automatic Compliance Schedule" of the acute
WET portion of the Colorado State Permit System Authorization to Discharge, SGS North
America Inc. conducted an accelerated one species acute bioassay on the Western Fuels New Horizon
Mine effluent outfall 011 discharge. The test organism utilized was the Pimephales promelas. The
results for the first sample of the accelerated testing schedule indicate this effluent sample was
not acutely toxic to P. promelas survival.
Methods
A one species accelerated acute bioassay was conducted with effluent from the Western Fuels New
Horizon Mine, outfall 011. An effluent grab sample was collected on September 29, 2008, stored on ice,
and delivered to SGS North America Inc. on the next day. The effluent sample was received at a
temperature of 1.00 C and assigned sample tracking number 411-080-BIOA-071. The bioassay was
initiated approximately 24 hours after sample collection.
Testing was conducted in accordance with the most recent protocols specified by the Water Quality
Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics
of the effluent and dilutions were measured by the methods and equipment outlined in Table 2.
Statistically, the Binomial method was used to determine the LC 50 2. Significant differences in mortality
were analyzed using Dunnett's test.
z Supplemental forms include statistical analysis and chain-of-custody forms
LC50 - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test