2008-10-22_REPORT - C1981008 ‎(3)‎

• Permit No: C1981008
• IBM Index Class Name: REPORT
• Doc Date: 10/22/2008
• Doc Name: 2008 AHR Appendix C Part 3
• From: Western Fuels-Colorado
• To: DRMS
• Annual Report Year: 2008
• Permit Index Doc Type: Hydrology Report
• Email Name: MLT
• Media Type: D
• Archive: No

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Table of Contents
Abstract
Methods
Results
Narrative & Discussion
Table 1 - Colo. Test Conditions
Abstract
Table 2 - Methods and Equipment
References
Discharge Monitoring Report Form
Reference Toxicity Data Chart(s)
Supplement Forms'
SGS North America Inc. conducted a two species acute bioassay on Western Fuels New Horizon Mine
effluent discharge. This discharge has been designated as outfall 011. The purpose of this bioassay
was to determine if the facility complies with the acute WET portion of the Colorado State Industrial
General Minimal Discharge Permit. The test organisms utilized were the fathead minnow (Pimephales
promeles) and cladoceran (Ceriodaphnia dubia). Test results indicate that the effluent was acutely
toxic to fathead minnow but was not acutely toxic to Ceriodaphnia dubia survival. Therefore,
outfall 011 may not be in compliance with the acute WET portion of its Colorado State Discharge
Permit for the third quarter of 2008.
Methods
A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine,
outfall 011. An effluent grab sample was collected on September 15, 2008, stored on ice, and delivered
to SGS North America Inc. on the next day. The effluent sample was received at a temperature of
10.5°C and assigned sample tracking number 411-080-BIOA-059. The bioassay was initiated
approximately 24 hours after sample collection. The client was contacted upon sample receipt, notified of
receiving temperature, and gave verbal okay to proceed with test initiation.
Testing was conducted in accordance with the most recent protocols specified by the Water Quality
Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics
of the effluent and dilutions were measured by the methods and equipment outlined in Table 2.
The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promeles, to
five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted
laboratory water- Control replicates were exposed to 100% laboratory water.
Statistically, the Binomial method determined the LC 50,S2. Significant differences in mortality were
analyzed using the Dunnett's Test and Fisher's Exact test.
' Supplemental forms include statistical analysis and chain-of-custody forms
z LCeo - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test
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