2008-10-22_REPORT - C1981008 (3)

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Western Fuels 011 Acute Pp August 2008 0Program run on 09-22-2008 at 12:02:12 RESULTS CALCULATED USING THE BINOMINAL METHOD CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB.(PERCENT) --------- 100 --------------- 40 -------------- 33 --------------- 82.50 --------------------------- 0.0021 75 40 20 50.00 56.2684 50 40 9 22.50 0.0340 25 40 2 5.00 LESS THAN 0.001 12.5 40 3 7.50 LESS THAN 0.001 THE BINOMIAL TEST SHOWS THAT -50 AND 100 CAN BE USED AS STATISTICALLY SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS BECAUSE THE ACTUAL CONFIDENCE LEVEL ASSOCIATED WITH THESE LIMITS IS GREATER THAN 95 PERCENT 75.00001 CAN BE USED AS AN APPROXIMATE LC50 FOR THIS DATA SET RESULTS CALCULATED USING THE MOVING AVERAGE METHOD -------------- -------- SPAN ----------------- G ---•--------------- LC50 ---------------- 95 PERCENT ------- CONFIDENCE LIMITS ------- 3 ------------------ 0.064 ------------------ 69.307 ---------------- 61.548 --------------------- 79.299 2 0.121 71.620 63.925 79.874 1 0.402 75.000 56.170 82.561 1 0.591 75.000 63.952 157.956 AN LC50 CALCULATED USING THE MOVING AVERAGE METHOD MAY NOT BE A VERY GOOD ESTIMATE IF THE SPAN IS MUCH LESS THAN THE NUMBER OF CONCENTRATIONS. IF ANY HIGHER CONCENTRATION PRODUCES A LOWER PERCENT DEAD THAN ALOWER CONCENTRATION, THE CONFIDENCE LIMIT AS OBTAINED BY THE MOVING AVERAGE METHOD WILL PROBABLY BE TOO CLOSE. RESULTS CALCULATED USING THE PROBIT METHOD -------------------------------------------------------------------------------- GOODNESS OF FIT ITERATIONS G H CHI-SQUARE PROBABILITY -------------------------------------------------------------------------------- 3 1.210 5.406 16.219 0.001 BECAUSE THE PROBABILITY IS LESS THAN 0.05, RESULTS CALCULATED USING THE PROBIT METHOD PROBABLY SHOULD NOT BE USED. '.SLOPE = 2.882167 95 PERCENT CONFIDENCE LIMITS = -.2887824 AND 6.053116 LC50 = 68.29831 95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY