2008-10-22_REPORT - C1981008 (2)

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• 0 • Table 1 - Colorado Chronic Test Conditions Parameter Test Type Temperature Photoperiod Test Chamber Size Test Sample Volume Renewal Period Age of Organisms Number per Chamber Chambers per Concentration Feeding Regime per Chamber Test Duration Ceriodaphnia dubla Static renewal 25 +/-1 ° C 16 hr light/8 hr dark 30 mL 20 mL 24 hrs < 24 hrs 1 10 0.1 ml Algae/YTC per day Until 60% of control organisms have 3 broods or 8 days Fathead Minnow Static renewal 25 +/- 1 ° C 16 hr light/8 hr dark 600 mL 300 mL 24 hrs < 24 hrs 10 4 0.15 ml Brine shrimp 2x/day. 7 days Table 2 - Methods and Equipment Characteristic Alkalinity Ammonia Chlorine, Total Residual Conductivity Dissolved Oxygen Hardness pH Temperature Analytical Balance Method/Equipment Titdmetdc CHEMetrics Colorimetery K-1510 CHEMetrics Colorimetery K-2504 Cole-Parmer Model 1500 Cond. Meter YSI Model 59 DO Meter Titrimetric Fisher Accumet Model 910 pH Meter NISr calibrated thermometer Mettler AE 240 EPA Method EPA 310.1 EPA 120.1 EPA 360.1 EPA 310.1 & 130.2 EPA 150.1 References Methods for Chemical Analysis of Water and Waste, Environmental Protection Agency, Environmental Monitoring Systems Laboratory - Cincinnati (EMSL-CI), EPA-600/4-79-020, Revised March 1983 Colorado Department of Health 1998 Guidelines for Conducting Whole Effluent Toxicity Tests Water Quality Control Division, Denver, Colorado 80246-1530. US EPA, October 2002, Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, Fourth Edition, U.S. Environmental Protection Agency, Office of Water, Washington, D.C. EPA-821-R-02-013 5 National Institute of Standards and Technology