2008-10-22_REPORT - C1981008 ‎(2)‎

• Permit No: C1981008
• IBM Index Class Name: REPORT
• Doc Date: 10/22/2008
• Doc Name: 2008 AHR Appendix C Part 2
• From: Western Fuels-Colorado
• To: DRMS
• Annual Report Year: 2008
• Permit Index Doc Type: Hydrology Report
• Email Name: MLT
• Media Type: D
• Archive: No

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Table of Contents
Abstract
Methods Page 2 Table 2 - Methods and Equipment Page 5

Results Page 2
Page 3 References
Discharge Monitoring Report Form(s) Page 5
Pg. 6-7
Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8
Table 1 - Colorado Test Conditions Page 5 Supplement Forms' Pg. 9
Abstract
SGS North America, Inc. conducted a two species chronic bioassay on Western Fuels New Horizon Mine
effluent discharge. This discharge has been designated as outfall 007. The purpose of this bioassay
was to determine if the facility complies with the chronic WET portion of the Colorado State Permit
System Authorization to Discharge. The test organisms utilized were the fathead minnow (Pimephales
promelas) and cladoceran (Cenodaphnia dubia). Test results Indicate that the effluent was not
chronically toxic to fathead minnow or to Ceriodaphnia dubia survival. Therefore, outfall 007 is in
compliance with the chronic WET portion of its Colorado State Discharge Permit for the first
quarter of 2008.
Methods
A two species chronic bioassay was conducted with effluent from Western Fuels New Horizon Mine,
Outfall 007. An effluent composite sample was collected on March 3, 2008, stored on ice, and delivered
to SGS North America Inc. on March 4, 2008. The effluent samples were received at a temperature of
4.3° C and assigned sample tracking number 411-OMBIOA-015. The bioassay was initiated
approximately 24 hours after sample collection.
Subsequent samples were collected on March 5, 2006, stored on ice, and delivered to SGS North
America Inc. on March 6, 2008. The effluent samples were received at a temperature of 3.1 °C.
Because of the remote location of the facility, two concurrent samplings "events were utilized for this
examination. Sufficient sample volume was collected for the entire test.
Testing was conducted in accordance with the most recent protocols specified by the Water Quality
Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics
of the effluent and dilutions were measured by the methods and equipment outlined in Table 2.
The tests exposed the cladocerans, Cedodaphnia dubia, and fathead minnows, Pimephales promelas, to
five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted
laboratory water. Control replicates were exposed to 100% laboratory water.
Statistically, fathead minnow survival and growth data were analyzed for significant differences using
Steel's Many One Rank Test and Dunnett's test, respectively. Cedodaphnia dubia survival and
reproduction data were analyzed for significant differences using Fishers Exact Test and Dunnet's Test,
respectively. IC262 values were determined using the Bootstrap Linear Interpolation method.
' Supplemental forms include statistical analysis and chain-of-custody forms
2 Inhibition Concentration at 25% reduction