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Part I <br />Permit No: CO-0000213 <br />Page 13a (replaces p13) <br />4a. Chronic WET Testing-Outfall(s): 007 <br />a. Testing and Reporting Requirements <br />Tests shall be-done at the frequency listed in Part I.B.1. Test results shall be reported along with the Discharge Monitoring <br />Report (DMR) submitted for the reporting period during which the sample was taken. (i.e., WET testing results for the fast <br />calendar quarter ending March 31 shall be reported with the DMR due Apri128.) The results shall be submitted on the <br />Chronic Toxicity Test report form, available from the Division. Copies of these reports are to be submitted to both the <br />Division along with the DMR. <br />The permittee shall conduct each chronic WET test in general accordance with methods described in Short Term Methods <br />for Estimatine the Chronic Toxiciri of Effluents and Receiving Waters to Freshwater Organisms EPA/600/4-89/001 or the <br />most current edition, except as modified by the most current Division guidance document entitled Guidelines for Conducting <br />Whole Effluent Toxicity Tests. The permittee shall conduct such tests using Ceriodaphnia dubia and fathead minnows. <br />b. Failure of Test and Division Notification <br />$eginning on the effective date of the limitation, a chronic WET test is failed whenever there is a statistically significant <br />difference in lethality between the control and any effluent concentration less than or equal to the instream waste <br />concentration (IWC). The IWC for this pemut has been determined to be 100%. The pemuttee must provide written <br />notification of the failure of a WET test to the Division, along with a statement as to whether a Preliminary Toxicity <br />Investigation (PTI)1Toxicity Identification Evaluation (TIE) or accelerated testing is being performed. Notification must be <br />received by the Division within 21 calendar days of the demonstration of chronic WET in the routine required test. <br />Demonstration for the purposes of Parts I.A.4.b., c., d., e. and g. means no later than the last day of the laboratory test. <br />4b. Acute WET testing - Outfall(s): 008, 009, 010, 011, 012 and 013 <br />a. Testing and Reporting Requirements <br />Tests shall be done at the frequency listed in Part I.B.1. Test results shall be reported along with the Discharge Monitoring <br />Report (DMR) submitted for the end of the reporting period when the sample was taken. (i.e., WET testing results for the <br />calendar quarter ending March 31 shall be reported with the DMR due Apri128, etc.) The results shall be submitted on the <br />Acute Toxicity Test report form, available from the Division. Copies of these reports are to be submitted to both the <br />Division and EPA along with the DMR. <br />The permittee shall conduct each acute WET test in genera] accordance with methods described in Methods for Measuring <br />the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms EPA/600/4-90/027 or its most <br />current edition, except as modified by the most current Division guidance document entitled Guidelines for Conducting <br />Whole Effluent Toxicity Tests. The permittee shall conduct an acute 48-hour WET test using Ceriodaphnia sp., and an <br />acute 96-hour WET test using fathead minnows. Acute tests will be replacement static tests of a single effluent grab sample. <br />b. Failure of Test and Division Notification <br />Beginning on the effective date of the limitations, an acute WET test is failed whenever the LC50, which represents an <br />estimate of the effluent concentration which is lethal to 50% of the test organisms in the time period prescribed by the test, is <br />found to be less than or equal to I00% effluent. The pemtittee must provide written notification of the failure of a WET test <br />to the Division, along with a statement as to whether the Preliminary Toxicity Incident (PTI)1Toxicity Identification <br />Evaluation (TIE) investigation or accelerated testing is being performed. Notification must be received by the Division <br />within 14 calendar days of the demonstration of acute WET in the routine required test. Demonstration for the purposes of <br />Parts I.A.3.b., c., d. and (f) means no later than the last day of the laboratory test. <br />AMENDED: OCTOBER 24 2007 EFFECTIVE: DECEMBER 1 2007 EXPIItATION: MARCH 31 2012 <br />Attachment 2.05.3(3)-18-16 <br />