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Enviro-Chem Analytical, Inc. Acute Toxicity Testing <br />685 West Gunnison Avenue, Suite 108 West Elk Mine - April 2008 <br />Grand Junction, CO 81505 Outfall 001 2 Species <br />Results <br />Effluent Chemistry Results <br />Table 1 contains the arrival chemistry values obtained for the sample. Effluent <br />sample arrival temperatures was 1.0 C. Total ammonia concentration was below <br />detection (<1.0 mg/L). The total residual chlorine was below detection (<0.02 <br />mg/L). <br />Tables 2 and 3 contain the routine chemistry results obtained during the toxicity <br />tests. All pH, dissolved oxygen, conductivity, and temperature values were <br />within acceptable ranges. <br />Effluent Toxicity Resufts for Fathead Minnows <br />Table 4 contains the survival results for the fathead minnow effluent test. <br />Control organism survival (100%) met the minimum performance requirement. <br />Acute survival results show that all effluent dilutions supported mean survival of <br />100%. The undiluted effluent supported mean survival of 100%. <br />The LC50 was >100%. <br />Effluent Toxicity Resufts for Daphnia magna <br />Table 5 summarizes the survival results for the Daphnia magna test. The acute <br />results show control organism survival met minimum performance requirements. <br />The MHW control exposure supported 100% survival. The effluent <br />concentrations all supported survivals of 100%. <br />The LC50 was >100%. <br />5