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Enviro-Chem Analytical, Inc. Acute Toxicity Testing <br />685 West Gunnison Avenue, Suite 108 West Elk Mine -Feb 2008 <br />Grand Junction, CO 81505 Outfall 001 2 Species <br />Table Of Contents <br />Study Summary ....................................................................................1 <br />Introduction ............................................................................................ 2 <br />Methods .................................................................................................2 <br />Sample Collection ................................................~----..................2 <br />Sample Preparation and Usage ..................................................3 <br />Test Performance ........................................................................3 <br />Test Acceptability .........................................................................4 <br />Statistical Analyses ......................................................................4 <br />Res u Its ..................................................................................................... 5 <br />Effluent Chemistry Results ............................................................5 <br />Effluent Toxicity Results for Fathead Minnows ...............................5 <br />Effluent Toxicity Results for Daphnia magna .....................................5 <br />References ................................................................................................6 <br />Table 1. Arrival Chemistry Values for Final Effluent Sample from West Elk <br />Mine Somerset, Colorado <br />Table 2. Initial Chemistry Ranges for Fathead Minnow and Daphnia magna <br />96-hour and 48-hour Acute Toxicity Tests Conducted with <br />West Elk Mine Effluent -Test Dates Feb. 26-29, 2008 <br />Table 3. Final Chemistry Ranges for Fathead Minnow and Daphnia magna <br />Toxicity Feb. 26-29, 2008 <br />Table 4. Percentage Survival Larval Fathead Minnows After 96-hour <br />Exposure to West Elk Mine Effluent -Test Dates <br />Feb. 26-29, 2008 <br />