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,Acute ~iotoxicity bench Sheet <br />Client: ~~ t~r7~ ~~~ ~-~~, p~ - <br />Project: Lab. No.: <br />Date Received <br />Sample Date: Time: Date Analyzed: <br />Source: Analyst: ~~ /~~ _ <br />-~,,_-~- <br />Source of dilution water: NIM~S~~J <br />Test Species: '~~ A9e~ <24 Hours Temp. Range: °C <br />Type of Test: tE~ ~~ ~t~~ <br />Total Chlorine: <br /> Beginning Ending <br />Date: 3 2 08 3 tL{ Od <br />Time: t~p~y !moo <br />Concentration Control d• l~Z~ / l ~ LS /~ 2 •S L S L. l o L <br />START <br />Temperature a; ! .~ 2 I.a Z t.a Z(,O 2t ,O 2 f .~ <br />Hardness ga, - ~ <br />D.o. ~t.5 .~{ .~, .o .9 8 ~ <br />pH ~. ~ '?. <br />Alkalinity 7 <br />Sp. Conduct. 3d~ D 0 t 2.t3o°i iSbF t~ i'p'3o <br />24 FFOUR <br />Temperature a.p..g a.p• 4' ~,a . 8' aO• ~ qtr. ¢ at d- ~ <br />No. Surviving 2p ~ 3-~ a.a ao ~ <br />48 HOUR <br />Temperature , 9 ~c~, °~ ~.a.°1 ~Lo.q ot,d. °- ao .h <br />No. Surviving y p ao a~ ~ o ~ o .~ <br />Note: All results expressed in mg/L unless otherwise designated. < =less than <br />Nate: Number in parenthesis equals number not adversely effected (ECso). This number is used in calculating <br />Aso value. <br />Note: Due to fragile structure of Daphnia organisms, dissolved oxygen (DO), hardness, alkalinity, specific <br />conductance, and pH reading could not be taken after the organisms are added to the sample. Doing so would <br />cause injury to the organisms. <br />Method Reference: Methods for Measuring fhe Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine <br />Organisms., Fifth Edition. EPA-821-R-02-012. U.S.EPA, Washington, DC. <br />f:\public\forms\bioassay\report2.doc <br />