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• For each area sampled, a minimum of 30 production transacts were used for sample adequacy <br />calculations. Following clipping, the harvested plant materials were dried in a drying oven at 100 <br />degrees C for a period of 24 hours. <br />Shrub Density. Starting at each of the 30 transact locations described above for the production <br />sampling, the fifty-meter tape was laid out along a predetermined and randomly selected <br />alignment. Woody stem density was determined using the center of the tape as a modified belt <br />transact. The right-hand side of the h~ansect was called Side A and the left-hand side of the tape <br />was called Side B. Data collected from each side of the tape were added into a transact value <br />which represents the number of shrubs sampled in the 100 m2 sample plot. These data are <br />reported in the Results Section as to the number of shrubs per 100 m2 as well as the number of <br />shrubs per acre. <br />Species Diversity. The plant cover data collected at each cover sample transact yielded data <br />relative to absolute and relative cover. These cover data were then converted into percent <br />composition or relative plant cover by species based upon the total amount of plant cover <br />encountered in each sample transact. The relative importance values of plants growing on the <br />reclaimed areas as outlined in the Permit Application, contain standards as to the number of <br />perennial grass species growing on the reclaimed areas to ensure that a monoculture of a single <br />plant or a few plants does not dominate the reclaimed areas. <br />As described in the proposed Northfield Mine Permit Application, the species diversity standard <br />• being proposed, is that at the Northfield Mine site, the reclaimed plant community shall be <br />composed of at least the number of dominant perennial species comprising at least five percent <br />of the relative plant cover. <br />Statistical Analysis of Data. As required by the Division's regulations and associated policies, <br />it is not necessary to achieve sample adequacy for vegetation monitoring, provided that a <br />maximum of at least fifty transccts have been sampled. If sample adequacy is achieved, a <br />minimum of a least 15 transacts for cover and 30 transacts for production and shrub density must <br />still be collected. Verbal approval to cease sampling after fifty transacts had been collected was <br />obtained from Ms. Sandy Brown and Mr. Dan Mathews during their site visit. Therefore, all of <br />the data collected in this evaluation were analyzed for sample adequacy in order to determine <br />whether the Division's requirements had been satisfied. The new sample adequacy formula <br />contained in the current regulations was used, wherein a precision of 0.10 was used for cover and <br />production and 0.15 for shrub density. <br />On the proposed disturbance areas and reference areas sampled, all of the field data were <br />analyzed in terms of "total plant cover" to determine whether sample adequacy was achieved <br />prior to leaving the site being sampled. On the areas sampled for production, a minimum of 30 <br />samples were collected and the green plant weight data analyzed to determine whether sample <br />adequacy had been achieved. On all sites sampled for shrub density, a minimum of 30 samples <br />were collected and the data analyzed to determine whether sample adequacy had been achieved. <br />In instances were pr~uction or shrub density was not achieved with 30 samples, then a <br />• 4 <br />