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<br /> <br />Enviro-Chem Analytical, Inc. <br />685 West Gunnison Avenue, Suite 108 <br />Grand Junction, CO 81505 Outfall 017 <br />2 Species <br />Acute Toxicity Testing <br />West Elk Mine -October 2007 <br />Table Of Contents <br />Study Summary ....................................................................................1 <br />Introduction ............................................................................................2 <br />Methods.... ..... ...........................................................................2 <br />Sample Collection .......................................................................2 <br />Sample Preparation and Usage ..................................................3 <br />Test Performance ........................................................................3 <br />Test Acceptability .........................................................................4 <br />Statistical Analyses ......................................................................4 <br />Res u Its .............. ............................................................................ 5 <br />Effluent Chemistry Results ............................................................5 <br />Effluent Toxicity Results for Fathead Minnows ...............................5 <br />Effluent Toxicity Results for Daphnia magna .................................5 <br />References........... .. ...........................................................................6 <br />Table 1. Arrival Chemistry Values for Final Effluent Sample from West Elk <br />Mine Somerset, Colorado <br />Table 2. Initial Chemistry Ranges for Fathead Minnow <br />96-hour Acute Toxicity Tests Conducted with T <br />West Elk Mine Effluent -Test Dates Oct. 23-27, 2007 <br />Table 3. Final Chemistry Ranges for Fathead Minnow and Daphnia magna <br />Toxicity Tests Conducted with West Elk Mine Effluent -Test Dates <br />Oct. 23-27, 2007 <br />Table 4. Percentage Survival Larval Fathead Minnows After 96-hour <br />Exposure to West Elk Mine Effluent -Test Dates Oct. 23-27, 2007 <br />