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<br />Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplemental Forms' Pg. 10 <br />Abstract <br />SGS North America Inc. conducted a two species acute bioassay on Western Fuels effluent discharge. <br />This discharge has been designated as ouffall 007. The purpose of this bioassay was to determine if the <br />facility complies with the acute WET portion of the Colorado State Permit System Authorization to <br />Discharge. The test organisms utilized were the fathead minnow (Pimephales promelas) and cladoceran <br />(Daphnia magna). Test results indicate that the effluent was not acutely toxic to fathead minnow <br />and Daphnia magna survival. Therefore, outfall 007 is in compliance with the acute WET portion <br />of its Colorado State Discharge Permit for the fourth quarter of 2006. <br />Methods <br />A two species acute bioassay was conducted with effluent from the Western Fuels, ouffall 007. An <br />effluent grab sample was collected on November 6, 2006, stored on ice, and delivered to SGS North <br />America Inc. on the next day. The effluent sample was received at a temperature of 4°C and assigned <br />sample tracking number 072-22844-001. The bioassay was initiated approximately 27 hours after <br />sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Daphnia magna, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water. Control replicates were exposed to 100% laboratory water. <br />' Statistically, the Binomial method was used to determine the LC~Z for both P. promelas and D. magna. <br />Significant differences in mortality were analyzed using the Steel's Many-One Rank test. <br />' Supplemental forms include statistical analysis and chain-of-custody forms <br />z LCs° - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test <br />