REP46588

• Permit No: C1981008
• IBM Index Class Name: Report
• Doc Date: 10/10/2006
• Doc Name: 2005 AHR Part 3
• From: Western Fuels-Colorado
• To: DRMS
• Annual Report Year: 2005
• Permit Index Doc Type: Hydrology Report
• Media Type: D
• Archive: No

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Table of Contents
Abstract
Methods
Results
Narrative & Discussion
Table 1 -Colo. Test Conditions
Page 2 Table 2 -Methods and Equipment
Page 2 References
Page 3 Discharge Monitoring Report Form
Page 4 Reference Toxicity Data Chart(s)
Page 5 Supplemental Forms'
Page 5
Page 5
Pg. 6-7
Pg. 8-9
Pg. 10
Abstract
SGS North America Inc. conducted a two species acute bioassay on Westem Fuels effluent discharge.
This discharge has been designated as outfall 007. The purpose of this bioassay was to determine if the
facility complies with the acute WET portion of the Colorado State Permit System Authorization to
Discharge. The test organisms utilized were the fathead minnow (Pimephales promelas) and cladoceran
(Daphnia Magna). Test results indicate that the effluent was not acutely toxic to fathead minnow
and Daphnia Magna survival. Therefore, outfali 007 is in compliance with the acute WET portion
f ifs Colorado State Discharge Permit for the second quarter of 2006.
Methods
A two species acute bioassay was conducted with effluent from the Western Fuels, outfall 007. An
effluent grab sample was collected on May 30, 2006, stored on ice, and delivered to SGS North America
lnc, on the next day. The effluent sample was received at a temperature of 8°C and assigned sample
tracking number 072-19819-001. The bioassay was initiated approximately 24 hours after sample
collection.
Testing was conducted in accordance with the most recent protocols specified by the Water Quality
Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics
of the effluent and dilutions were measured by the methods and equipment outlined in Table 2.
The tests exposed the cladocerans, Daphnia Magna, and fathead minnows, Pimephales promelas, to
five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted
laboratory water. Control replicates were exposed to 100% laboratory water.
Statistically, the Binomial method was used to determine the LC~oZ for both P. promelas and D. magna.
Significant differences in mortality were analyzed using the Steel's Many-One Rank test.

' Supplemental forms include statistical analysis and chain-of-custody forms
z LCso - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test
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