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•SG9, <br />Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplemental Forms' Pg. 10 <br />Abstract <br />SGS North America Inc. conducted a two species acute bioassay on Western Fuels effluent discharge. <br />This discharge has been designated as ouffall 008. The purpose of this bioassay was to determine if the <br />facility complies with the acute WET portion of the Colorado State Permit System Authorization to <br />Discharge. The test organisms utilized were the fathead minnow (Pimephales promelas) and cladoceran <br />(Ceriodaphnia dubia). Test results indicate that the effluent was not acutely toxic to fathead <br />minnow and Ceriodaphnia dubia survival. Therefore, outfall 008 is in compliance with the acute <br />WET portion of its Colorado State Discharge Permit for the third quarter of 2006. <br />Methods <br />A two species acute bioassay was conducted with effluent from the Western Fuels, ouffall 008. An <br />effluent grab sample was collected on September 5, 2006, stored on ice, and delivered to SGS North <br />America Inc. on the next day. The effluent sample was received at a temperature of 13°C and assigned <br />sample tracking number 072-21623-001. The bioassay was initiated approximately 26 hours after <br />sample cotlection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water. Control replicates were exposed to 100% laboratory water. <br />Statistically, the Binomial and Probit methods were used to determine the LCS Zfor both P. promelas and <br />C. dubia. Significant differences in mortality were analyzed using the Steel's Many-One Rank test. <br /> <br />~ Supplemental forms include statistical analysis and chain-of-custody forms <br />z LCw - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test <br />2 <br />F-526 <br />