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PART I Page if of 19 Permit IJo. CO-0000213 B. MONITGRL~G REQUIREMENTS 2. Frequency and Sample Type - ?cute WET Testing - Outfe~ll 001 The: monitoring frequency for acute WET tests shall be quarterly, co¢imencing with the first full calendar quarter following the permit. effective date. Quarterly test results shall be reported along with the Discharge Monitoring Report CONK) submitted for the end of the reporting calendar quarter Ci.e., WET testing results for the ~:alendar quarter ending March 31 shall be reported with the D!1P: due Aril 28, with the remaining WET testing reports submitted with I)MRs du.z each July 28, October 28 and January 28). The results shall be submitted oa the Acute Toxicity Test report form, available from the Division. Copies of these reports are to be submitted to both the Division and EPA. Thee permittee shall conduct each acute WET test in general accordance with methods described in the Division guidance document entitled Guidelines for Conducting Whole Effluent Toxicity Tests. The permittee shall conduct an acute 48-hour WET test using Cerioda hnia sp., and an acute 96-hour WET test using fathead minnows. Acute tests will be replacement static tests of a single effluent grab sample. 3. Accelerated Testin If an individual toxicity test shows toxicity at concentrations less than or equal to the Instream Waste Concentration C]NC) of 31A or if 50'S species mortality in any dilution is detected is any acute WET test conducted in accordance with this permit, the permittee shall notify the Division verbally xithin 24 hours and is writinL; within S days of becoming aware of the toxicity. Within 7 days of becoming aware of the toxicity, the permittee shall then begin accelerates. testing'of the discharge with whichever species has proven (throug}~ the first instance of toxicity) to be the most sensitive. The permittee will continue such testing with one species, once every week until. directed by the Division to do other,+ise, but in no case for more than five representative tests. 4. Preliminary Toxicity Investigation If directed by the Division, the permittee stall perform a preliminary toxicity investigation. The permittee will Have IS working days from t!~.e date of Division notification to complete a preliminary toxicity itvestigation and submit the results to the Ulvlsion, in writing. The preliminary toxicity investigation may include, but 1s not limited to: additional chemical and biological monitorial;, examination of pretreatment program records, examination of discharge monitoring reports, evaluation of treatment processes and chemical use, laspectlo^ of material storage and transfer areas to determine if a spill may have occurred, and similar procedures. Amended ;i?5inrt Effective=~"c'9p Revised S/2/89 i