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n ';:~.E. T. Inc. <br />WrsSer~& Environmenta! Testing Inc. 479 West 50 Norrh, American Fork Utah 84003 (8011756-R397 <br /> <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: June 25, 1996 <br />PERMTCCEE NAME: Western Fuels-Colorado (New Horizon Mine) NPDS NO: <br />TEST (Animal/Age): Fathead Minnow 11 days. <br />OUTFACE NO: 007 <br />SAMPLE (Da[e/Type): 6/19/96. <br />DATE/f1ME TESL' BEGAN: 6/20/96 1:30 p.m. DATE(I'Q4IEE TEST COMPLETED: 6/24/9b 2:00 p.m. <br />TEST COIYDIT[ONS <br />Fathead Minnow Larvae were exposed to diluted effluent as specified by permit. Survival at the end of the <br />lest period was measured and slatisticallyevalualed for Acute Toxicity. <br />n <br />L_J <br />11 days.: '. ~ i <br />5 organisms/200 ml/4 rcplicatcs. <br />Brine:Shrimp fed previous.to test .and :ai 48:hoots. <br />None required. <br />Measure daily.old/new..:: :: ; ::.; <br />Renewed every 24 hours. <br />r .:.:.:.::.:.:..::~.::;,.,:. .~.. <br />30: + 1 degrees C . .: :.:..: ........::.:.::::::. - ; ::.::,: <br />... <br />16 hours ambient light/8 hours dark. <br />Measuied initiallyand:ar24 hours:... _: ::;..::;:';_.'::.:;:jl::.::'' <br />Reconstituted lab water L+15% hardness of the i <br />Receiving Water). I <br />CFlatnl)y.I)taw: (Historical:Dala),::.'.: `.:':'S:' :> i..": ;:: ;.;, ::::;'..'. ;~ <br />1 :::: :..:..:...:..........:....:.:. ::.. <br />Control, 6.25%, 12.5%, 25%, SO%, 100%. <br />Dilution Water <br />Receiving' Watec ::...:: ::::: : : ::::.::.:.:..;..:'.:. : ;:.:: ::.. :... <br />,. <br />Sample Concentrations <br />SUMMARY <br />Results: X :. Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincere) <br />e Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />u <br />