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r - W.E. T. Inc. <br />Water & Environmental Testing Inc. 479 West 50 North. American Fork Utah 84003 (8 011 756-839 7 <br />~J <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: October 21, 1996 <br />PERMITTEE NAME: Western Fuels-Colorado (New Horizon Mine) NPDES NO: OUTFACE NO: 007 <br />TESL' (Animal/Age): Ceriodaphnia <24 hours. SAMPLE (Date/Type): 10/10/96 gab. <br />DATE/1'IME TESL' BEGAN: 10/11/96 6:00 p.m. DATE/1'Il1~ TEST C0117iPLETED: 10/13/96 7:00 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted effluent as specified by permit. Survival at the end <br />of the test period was measured and stalisticallyevalualcd for Acute Toxicity. <br />r 1 <br />L_.J <br />Auimal Age at Test:Start: .: <br />,...: . <br />y24 hours, :..:: ..;;"~..:; :: <br />a ..::...............:..:. <br />Number of Organisms/Dilution Volume/Replicates 5 organisms/15 ml/4 replicates. <br />Fuod ..:.':. ? ; ::.:::... :.:: 1^fC. with Algao (Ol ml' .......... .. . . <br /> <br />lday)(fed previous lo. test).: ; ::. <br />i <br />Aeration None required. <br />Dissolved::0irygen.::..::. ' ::::.:.::..:: ::.:. <br />...... :Measuieddaily:old/new;::.':." ...: :::: :::;:::'::::..'::'::::;:; <br />Water Replacement Renewed every 24 hours. <br />~'emperatuie., .;.,.,., , ..::: .. 20 ±:1 degees C:.; .: ,:. ~ ' .,.:: :..:.. .::.::.; ;;: ; , , <br />,.. <br />Photo Period 16 hours ambient 1ig41/8 hours dark. <br />p~.:::: :.::::;:::::'.::..';::.:,:: ;:'''.:::::::.: .:' ..:.: .:Measured inilt...lyand ai;~:.:;liouis:.::.:;..' ; :::;''.:.: <br />Dilution Water Reconstituted lab water L15% hazdness of the <br /> Receiving Water). <br />Receiv .:. Water :::::.:::.:::.:.: ..::..:.::.....:':;::..:..::.::: <br />............ . Calamtiy,Acaw::.::~storiCal flora);::::::::::;:::.>.::::;:;::::::.! .: <br /> <br />:..::..t ::.:...:.:... . <br />Sample Concentrations Control, 6.25%, 12.5%, 25%, 50%, 100%. <br />SUMMARY <br />Results: '::.}[:::;: Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />JInCe7e~ '~ A <br />Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />• <br />