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"~W.~. T. Inc. <br />-Water & Environmen[a! Tes6rre Inc. 479 West SO NoRh. American Fork Utah 84003 (80/)756.8397 <br />r~ <br />U <br />Acute Whole Effluent Toxicity Report <br />Ceriodanhnia <br />DATE: September 13, 1996 <br />PERMTI'I'EE NAME: Wcstem Fuels-Colorado (New Horizon Mine) NPDES NO: OVCFALL NO: 007 <br />TESL' (AnimaUAge): Ceriodaphnia <24 hours. SAMPLE (Dare/Type): 9/D4/96. <br />DATE/TIME TEST BEGAN: 9/05/96 4:00 p.m. DATE/I'17vIE TESL' COMPLhTED: 9!07/96 4:00 p.m. <br />TEST CONbITIONS <br />Ceriodaphttia dubia neonates were exposed to diluted effluent as specified by perc~it. Survival at the end <br />of the test period was measured and slatisticallyevaluated for Acute Toxicity. <br />U <br />Animal Age at Test Start . f :<24 hours <br />Number of Organisms/Dilution Volume/Replicates 5 organisms/15 m1/4 replicates. <br /> <br />:food :::::.:: '..'.:;::.!:.. :::.: • . ....:. YT'C with: Al e D 1 m da fed revidus. f0: test <br />.U .YX.... p )r.;;' <br />&9.. ~ '. : <br />: <br />. . <br /> <br />Aeration . : <br />. <br />:... <br />. . <br />4 : <br />None re aired. <br />;Dissolved en.:.; : ., . :;.:.: -' .. <br />Oxyg <br />Measured:daily'oldlueSa:::, `:.::,.:>"•:: .:,r: :...:.::.::::.::::. <br />Water Replacement Renewed every 24 boors. <br />,.. .. . <br />Temperatui!e.::. ::r ::...:.: ... :. <br />20 ± 1 degecs C.:...:: ::;;:::: ; ::..".. ;::: '; :. ;'i: <br />,... ... <br />Photo Period 16 hours ambient light/8 hours dark. <br />,:.... <br />. , :. <br />pg :.:.. <br /> <br />,...:. <br />Measured initi:i{l an ' <br />y... d ai 24houis. <br />. <br />Dilution Water Reconstituted lab water (±15% hardness of the <br /> Receiving Water). <br />Receiving. Water .:: .. ...:...:.::.. Calamity Dtaw..(Historic~l.data):.: `.' `•`: <br />Sample Concentrations Control, 6.25%, 12.5%, 25%, 50%, 100%. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant elYect on survival. <br />Eoclosyd arc data sheets and statistical reports. <br />.., , <br />~~ ~a <br />iwlings <br />& Environmental Testing, Inc. <br />~ Enclosure <br />1~ <br />