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W.E. T. Inc. <br />•{Yater & Environmental Testine Ine. 479 West SO Norih. American Fork Utah 84003 (801)756-8397 <br />r~ <br />Acute Whole Effluent Toxicity Report <br />Ceiiods~hnia <br />DATE: March 13, 1996 <br />PERMITTEE NAME: Western Fuels-Colorado (New Horimn Mioe) NPDES NO: <br />TEST (AninwilAge): Ceriodaphnia <24 hours. <br />OUTFACE NO: 007 <br />SAMPLE (DafelType): 3/07/96. <br />DATE/I'D1~ TESL BEGAN: 3/08/96 6:45 p.m. DATE/['D1~lE TEST COMPLETED: 3/10/96 6:15 p.m. <br />TEST CONDTI'IONS <br />Ceriodaphnia dubia neonates were exposed to diluted effluent as specified by permit. Survival at the eod <br />of the test period was measured aad statistically evaluated for Acute Toxicity. <br /> <br />Animal Age. at Test Start <24 hours. ;; <br />~..... <br />Number of Orgaoisms/Dilution Volume/Replicates 5 organisms/15 ml/4 replicates. <br />,. <br />Tt'oad :::.:::.:: <br />YTC with Algae (0.1. ml/day)(fed pievious io test). '.: <br />Aeration None required. <br />Dissolyed.Ozygen :..::: Measured:daily:old/new...::..::.:::::::: :::..:::::: .: <br />Water Replacement Renewed every 24 hours. <br /> <br />TemperaWre...:... .: ......... <br />20 f S degrees. C,..:..: t ...::.::::. ..:......... .... <br />Photo Period 16 hours ambient light/8 hours dazk. <br />,. <br /> <br /> <br />.. :........ <br />,:. ,. <br />..., .. <br /> <br /> <br /> <br />; :..:.:.::.:..:. . <br />:M tiailyana.at24houirs <br />ensured: tm <br />,. <br />Dilution Water Reconstituted lab water (±15% hardness of the <br /> Receiving Water). <br />~ .. , ' . <br />': <br /> <br />S y ........~ ..., <br /> <br />cal. daW)~:`:::::::..:' ::::::..:::.::'. ' <br />tro <br />ample Coo ntrations <br />25%, 50%, 100%. <br />l, 6.25%, 128% <br />Con <br />SUMMARY <br />Results: 7C Pus Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets aad statistical reports. <br />~L <br />Water & Environmental Testing, Inc. <br />Enclosure <br />