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W.E. T. Inc. <br />Water & Environmertlal Testing /nc. 479 West SO North. American Fork Utah 84003 (801)756-8397 <br />C ~ <br />J <br />Acute Whole Effluent Toxicity Report <br />Ceriodanhnia <br />DATE: ]une 25, 1996 <br />PERMITTEE NAME: Western Fuels-Colorado (New Horizon Mine) NPDES NO: <br />TESL' (AnimallAge): Ceriodaphuia <24 hours. <br />OUTFACE NO: 007 <br />SAMPLE (DatelType): 6/19/96. <br />DATE/T11~iE TEST BEGAN: 6/20/96 1:45 p.m. DATE/I'1ME TEST COMPLETED: 6/22/96 2:00 p.m. <br />TEST CONDITIONS <br />C;eriodaphnia dubia neonates were exposed to diluted efYlucot as speciticd by permit. Survival al the end <br />of the test period was measured and statistically evaluated for Acute Toxicity. <br />• <br />Animal Age.at:Test.Start: :.: <24: hours: <br />Number of Organisms/Dilution Volume/Replicates 5 organisms/15 ml/4 replicates. <br />.. <br />. <br />Fdad ::;::: ,.: <br />YTC with Algae:(0.1 ml/day)(fed:previous to test)::': <br />Aeration Noue required. <br />Dissolved:OxYBen.:::, :.::- .. .::...:::..: Measured:daily old/new: l :: :::..:...c :.::. ; ;'; :::....:. <br />_.. . <br />Water Replacement Renewed every 24 hours. <br />Temperature, :.:: :' ::...:...: ..: <br />..... 20:±:1 degrees, C,: ::f.-:;, .... ::':.:'::.:.::.:::.. :;': <br />Photo Period 16 hours ambient light/8 hours dark. <br /> Measpred iailiallyaad:al 24;hours.:.::.:.. :' .:::..:;... <br />Dilution Water Reconstituted lab water (±15% hardness of the <br /> Receiving Water). <br /> <br /> <br /> <br />Rec... ...Water.;.::.:' :.:.:::.....:::'.:.:.::..: ' ... <br />eivl <br />°~.. . <br />.... <br /> <br />. Calamity. Draw.:.(Ilistoncal data).:::.:;:..::::.::: ":::::....: ':: <br />Sample Concentrations Control, 6.25%, 12.5°!0, 25%, 50%, 100%. <br />SUMMARY <br />Results: }C:: Pass Fail <br />There was NO significant effect on survival. <br />Enclosed arc data sheets and statistical reports. <br />Sincere , <br />/y .L <br />Ra ings <br />ater & Environmental Testing, Inc. <br />Enclosure <br />