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~G~'~ <br /> <br />~J <br />Narrative and Discussion <br />PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water: <br />Receiving temperature(s): <br />CO-0000213 <br />Effluent Grab <br />12/13/05 0900 <br />12/14/2005 1225 <br />Reconstituted Laboratory Water <br />4.0°C <br />• TEST ORGANISMS <br />Fathead minnow <br />Ceriodaphnia dubia <br />Age: 5 days <br />Age: <24 hrs <br />• PROCEDURE <br />Acute, 48 and 96-hour bioassays as outlined in the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998). <br />• DISCUSSION <br />According to the Western Fuels -Colorado discharge permit (CO-0000213), an acute WET test is <br />failed whenever the LCso. which represents an estimate of the effluent concentration which is <br />lethal to 50% of the test organisms in the time period prescribed by the test, is found to be less <br />than or equal to 100% effluent. <br />By this definition, the results of this bioassay indicate that effluent from outfall 008 is not acutely <br />toxic to fathead minnow or Ceriodaphnia dubia survival. Therefore, outfall 008 is in compliance <br />with the acute WET portion of its Colorado State Industrial General Minimal Discharge Permit for <br />the fourth quarter of 2005. <br />• DATE OF REPORT <br />December 20, 2005 <br />Report prepared by: Regina Mahan <br />~~~.~,,~, ~2-2o-oS <br />Biomo oring Technician Date <br />Report reviewed by: Tony Stoyko <br />%e~n~i/~~ /~l~U2tI•D~ <br />Bior~n n~itorin upervisor Date <br />4 <br />F-52fi <br />