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.~ <br />Narrative and Discussion <br />• PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample: <br />Date and time of test start: <br />Dilution water. <br />Receiving temperature: <br />CO-0000213 <br />Effluent Composite <br />4/19/2006 0930 <br />4/20/2006 1250 <br />Reconstituted Laboratory Water <br />1°C <br />• TEST ORGANISMS <br />Fathead minnow Age: 12 days <br />• PROCEDURE <br />Acute, 96-hour bioassays as outlined in the most recent protocols spedfied by the Water Quality <br />Control Division of the Colorado Department of Health (1998). <br />• DISCUSSION <br />According to the Western Fuels -Colorado discharge permit (CO-0000213), an acute WET test is <br />failed whenever the LCD, which represents an estimate of the effluent concentration which is <br />lethal to 50% of the test organisms in the time period prescribed by the test, is found to be less <br />than or equal to 100% effluent. <br />To determine if the facility complies with the "Automatic Compliance Schedule" of the <br />acute WET portion of the Colorado State Permit System Authorization to Discharge, SGS <br />North America Inc. conducted an accelerated one specie acute bioassay on the Westem Fuels <br />effluent discharge. The test organism utilized was the fathead minnow (Pimephales promelas). <br />The results for the first sample of the accelerated testing schedule indicate this effluent <br />sample was not acutely toxic to fathead minnow survival. <br />• DATE OF REPORT <br />May 18, 2006 <br />• Report pre/p,,.a~red by: Tony Stoyko <br />/c'~A'I~"` x./8.07 <br />Biom nitorin Supervisor Date <br />4 <br />