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•~- <br />Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Page 6 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Page 7 <br />Table 1 -Colo. Test Conditions Page 5 Supplemental Forms' Page 10 <br />Abstract <br />In March 2006, a two species acute bioassay was conducted with effluent from the Western <br />Fuels, outfall 007. The purpose of the bioassay was to determine if the facility complies with the acute <br />WET portion of the Colorado State Permit System Authorization to Discharge. <br />According to the Westem discharge permit (CO-0000213), an acute WET test is failed whenever the <br />LCD, which represents an estimate of the effluent concentration which is lethal to 50% of the test <br />organisms in the time period prescribed by the test, is found to be less than or equal to 100% effluent. <br />Results of the testing indicated the sample was acutely toxic fo fathead minnow survival but not <br />fo Daphnia magna survival. <br />To determine if the facility complies with the °Automatic Compliance Schedule" of the acute <br />WET portion of the Colorado State Permit System Authorization to Discharge, SGS North <br />America Inc. conducted an accelerated one specie acute bioassay on the Western Fuels effluent <br />discharge. The test organism utilized was the fathead minnow (Pimephales promelas). The results for <br />the first sample of the accelerated testing schedule indicate this effluent sample was not acutely <br />toxic to fathead minnow survival. <br />Methods <br />A one specie accelerated acute bioassay was conducted with effluent from the Western Fuels, ouffall <br />007. An effluent grab sample was collected on April 19, 2006, stored on ice, and delivered to SGS North <br />America Inc. on the next day. The effluent sample was received at a temperature of 1° C and assigned <br />sample tracking number 072-19111-001. The bioassay was initiated approximately 27 hours after <br />sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />Statistically, the Binomial method was used to determine the LC~2 for P. promelas. Significant <br />• differences in mortality were analyzed using the Steel's Many-One Rank test. <br />' Supplemental forms include statistical analysis and chain-of-custody forms <br />Z LCso - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test <br />