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End Date: 4/2/2006 11:30 Lab ID: CO-00013-SGS North Americ Sample Type: DMR-Discharge Monitoring Report <br />Sample Date: 3/28!2006 10:17 Protocol: ACUTE-EPA-821-R-02-012 Test Species: PP-Pimephales promelas <br />12.5 1.0000 1.0000 1.0000 1.0000 <br />25 0.8000 0.9000 1.0000 1.0000 <br />50 0.0000 1.0000 0.8000 0.6000 <br />75 0.1000 0.6000 0.8000 0.9000 <br />100 0.3000 0.3000 0.3000 0.7000 <br />Conc--% <br />N-Mean <br />ntrol 0.9750 1.0000 1.0977 1.0472 1.2490 9.194 4 1.0000 1.0000 <br />12.5 1.0000 1.0256 1.0472 1.0472 1.0472 0.000 4 1.0000 1.0000 <br />25 0.9250 0.9487 1.1126 1.0472 1.2490 8.558 4 1.0000 1.0000 <br />50 0.6000 0.6154 0.8910 0.5236 1.1071 29.419 4 0.7500 0.7500 <br />75 0.6000 0.6154 0.8910 0.3218 1.2490 45.772 4 0.7500 0.7500 <br />100 0.4000 0.4103 0.6825 0.5796 0.9912 30.147 4 0.2500 0.2500 <br />Shapiro-Wilk'S TOSt indi0ate5 normal tli6tribOtiOn (p > 0.01) 0.89511 0.884 -0.8287 2.41013 <br />Equality of variance cannot be confirmed <br />Linear Interpolation (200 Resamples) <br />• Point % SD 95% CL(Exp) Skew <br />IC50 87.500 <br />1.0 <br />0.9 <br />0.8 <br />0.7 <br />q 0.6 <br />c <br />o°. 0.5 <br />N <br />~ 0.4 <br />0.3 <br />0.2 <br />0.1 <br />0.0 <br /> <br />0 50 100 150 <br />Dose <br />Page 1 ToxCalc v5.0.23 RcviowcA by ~!~"[ <br />