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WESTERN FUELS Pp <br />Program run on 03-26-2007 at 11:30:36 <br />;ULTS CALCULATED USING THE BINOMINAL METHOD <br />CONC. NUMBER NUMBER PERCENT BINOMIAL <br />EXPOSED DEAD <br />- <br />--- DEAD PROB .{PERCENT) <br />---- <br />---- <br />-------------------------- <br />100 40 1 ------------- <br />2.50 --------- <br />LESS ------ <br />THAN -------------- <br />0.001 <br />I <br />75 40 0 0.00 LESS THAN 0.001 <br />50 40 0 0.00 LESS THAN 0.001 <br />25 40 0 0.00 LESS THAN 0.001 <br />I <br />12.5 40 1 2.50 LESS THAN 0.001 <br />AT A CONFIDENCE LEVEL OF 95 PERCENT, THE BINOMIAL TEST <br />' SHOWS THAT THE LC50 IS ABOVE 100 <br />THE USEFULNESS OF ANY LC50 CALCULATED FROM THIS SET OF DATA <br />IS QUESTIONABLE BECAUSE A CONCENTRATION-EFFECT RELATIONSHIP <br />HAS NOT BEEN DEMONSTRATED OVER A REAS ONABLE RANGE (e.g. <br /><37 TO>63 OF PERCENT DEAD <br />NEITHER THE APPROXIMATE LC50 CALCULATION NOR THE MOVING <br />AVERAGE METHOD CAN BE USED WITH THIS SET OF DATA. <br />EITHER THE HIGHEST CONCENTRATION KILLED LESS THAN 50 <br />PERCENT OR THE LOWEST KILLED MORE THAN 50 PERCENT. <br />IF THE PROBIT SLOPE IS NEGATIVE, ENTER THE DATA AGAIN <br />' USING THE NUMBER ALIVE INSTEAD OF THE NUMBER DEAD. <br />,ULTS CALCULATED USING THE PROBIT METHOD <br />GOODNESS OF FIT <br />ITERATIONS G H CHI-SQUARE PROBABILITY <br />-------------------------------------------------------------------------------- <br />4 58.471 1.000 3.089 0.378 <br />SLOPE _ -.2025097 <br />95 PERCENT CONFIDENCE LIMITS = -1.751021 AND 1.346002 <br />LC50 = 1.258924E-10 <br />' 95 PERCENT CONFIDENCE LIMITS = 0 AND 1.90749 <br />LC1 = 38.66798 <br />95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY <br />COMPARE RESULTS WITH ORIGINAL DATA TO SEE IF THEY ARE <br />REASONABLE. <br />