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,W ~'. T. Inc. <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: March 10, 1997 <br />• <br />PERMITTEE NAME: Western Fuels Colorado SIGHT ID: New Horizon Mine <br />TEST (AnimaUAgeJ: Fathead Minnow, 6 days. <br />DATE/1'IME TEST BEGAN: 3/4/97 8:00 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted eflluen[ as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity waz present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilutioo Volume/Replicates <br />Food <br />OUTFACE NO: 007 <br />SAMPLE (Date//)~peJ: 3/3/97. <br />DATE/1'IME TEST COMPLETED: 3/8/97 8:10 p.m. <br />Fathead Minnow, 6 days. <br />10 organisms/I50 mL/2 replicates <br />Fed before starting test and a[ 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. , <br />20 ± I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and a[ 24 hours. <br />Reconstituted water (+I S% hardness of the receiving <br />water). <br />None was supplied. <br />Aeration <br />Dissolved Orygeu <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Sam le Concentt <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincer y, ~n ~/I <br />/~~(,cJ ~/I// <br />Lee Rawlings / <br />Water & Environmental Testing, lnc. <br />• Enclosure <br />