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W.E.T. Inc. <br />~r & Environmental Tesline /nc. X79 Wes! SO North, American Fork Ulah 8003 /8011756-8397 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: October 2, 1997. <br />• <br />PERMITTEE NAME: Western Fuel Colorado SICHT ID: New Horizon Mine <br />TEST (Animol/Age): Ceriodaphnia Q4 hours. <br />OUTFACE NO: 007 <br />SAMPLE (Date/Type): 9/26//97 Grab. <br />DATE/TIME TEST BEGAN: 9/27/97 5:40 p.m. DATE/1'IME TEST COMPLETED: 9/29/97 5:30 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At [he end of the test period Survival <br />was measured to determine if Acu[e Toxicity was present in the sample. <br />Animal Age at Test Start Q4 hours. <br />Number otOrganisms(Dilution Volume/Replicates 5 organisms/IS mW4 replicates. <br />Food Fed YTC and Algae before starting test. <br />Aeration None requved. <br />Dissolved Oxygen Measured daily old/new. <br />Water Replacement Renewed daily. <br />Temperature 20+ I degrees C. <br />Photo Period 16 hours light 8 hours dark. <br />pH Measured initially and at 24 hours. <br /> Reconstituted water (±I S% hardness of the receiving <br />Dilution Watcr water). <br />Receiving Water None was supplied. <br />Sample Concentrations- _ Control, 6.25 12.5 25 50 100%. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed aze data sheets and statistical reports. <br />Sin ly, <br />~~ ' <br />Lee Rawlings <br />Water & Environmental Testing, Inc. <br />• Enclosure <br />