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~.E: T. Inc. <br />Water & Environmental Testin¢ lnc. 479 West 50 North. American Fork Utah 84003 (8011756-8397 <br />~ Acute Whole Effluent Toxicity Report <br />~hR~ <br />DATE: October 21, 1996 <br />PERMIT"I'EE NAME: Western Fuels-Colorado (New Horimu Miue) NPDES NO: OIPI'FALL NO: 007 <br />TESL' (AnimallAge): Ceriodaphnia <24 hours. SAMPLE (Date/Type): 10/10/96 grab. <br />DATE/I'IME TESL BEGAN: 10/11/96 6:00 p.m. DATE/fBVIE TESL' COMPLETED: 10/13/96 7:00 p.m. <br />TEST CONDTI'IONS <br />Ceriodaphnia dubia neonates were exposed to diluted effluent as specified by permit. Survival at the end <br />of the les: period was measured and slatisticallyevaluatcd for Acute Toxicity. <br />• <br />Animal.Age at:Tcst $tarl. <24 hours:';' <br />., <br />Number of Organisms/Dilution Volume/Replicates 5 organisms/15 ml/4 replicates. <br />'Food :' :. .:: .. YT'C with Algae (0.1 ml/day)(fed pievious to: lest)::..: <br />Aeration Noae requited. <br /> <br />Dissolved Ox en <br />YS <br />Measured: dail old/sew: ~ . ; : .: . <br />Y <br />Water Replacement Renewed every 24 hours. <br />:Temperature ..... .. .::.::.. 20:+ 1..degtees. C.:.': „ . '::: ';.: <br />~... <br />:.. <br />Photo Period 16 hours ambient light/8~hours dazk. <br /> <br />:... Measured .initial] and.a1~24hours. . <br />Y <br />Dilution Water Reconstituted lab water (±15% hardness of the <br /> Receiving Water). <br />Receiving .R'atcr ., .: Calamity A[aw (HistOrical.da]a).:.. .. <br />•. <br />Sample Concentrations Control, 6.25%, 12.5%, 25%, 50%, 100%. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistics! reports. <br />amcere ! ~ <br />quJ ~//r///M,oJ6/ (_ <br />Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />