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W.E.T. rnc. <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: June 23, 1997. <br />PERMITTEE NAME: Western Fuel Colorado SIGHT ID: New Horimn Mine <br />TEST (AnimaUAge): Ceriodaphnia <24 hours. <br />OUTFACE NO: 007 <br />SAMPLE (Dare/TypeJ: 6/17//97 Grab. <br />DATE/1'IME TEST BEGAN: 6/18/97 8:30 p.m. DATE/TIME TEST COMPLETED: 6/20/97 8:30 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. , <br />Animal Age at Test Start <br />Number otOrgaaisms/Dilution Volume/Replicates <br />Food <br />• <br />Aeration <br />Dissolved Orygea <br />Water Replacement <br />Temperature <br />Photo Period <br />Q4 hours. <br />5 organisms/15 mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. , <br />Measured daily old/new. <br />Renewed daily. <br />20 + 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and a[ 24 hours. <br />Reconstituted water (+I 5% hardness of the receiving <br />water). <br />None was supplied. <br />pH <br />Dilution Water <br />Receiving Water <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed aze data sheets and statistical reports. <br />Sincer , <br />`~ 'n~~~ <br />n~~ y - -/ <br />ee Rawlings <br />• Water & Environmental Testing, Inc. <br />• Enclosure <br />