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' .W E. T. Inc. <br />Wa/er & Erivironmen(a/ TesNn¢ Inc 479 West SO Nor(h. American Fork Ulah 8400) /8011758397 <br />Acute Whole Effluent Toxicity Report <br />Fa(head Minnow <br />PERMITTEE NAME: Western Fuels Colorado SIGHT ID: New Horizon Mine <br />TEST (Animal/Age): Fathead Minnow, 7 days. <br />DATE/I'IME TEST BEGAN: 6/18/97 9:00 p.m <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed [o diluted effluent as specified by [he permit. At [he end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Orgaoisms/Dilutioo Volume/Replicates <br />Food <br />Aeration <br />Dissolved Orygev <br />• Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Sam IeConcentrati <br />SUMMARY <br />~l/ ~ <br />~~ b~~ <br />,Q~y2s~ ,` <br />~; . <br />•1 <br />DATE: Juue 23, 1997 <br />OUTFACE NO: 007 <br />SAMPLE (Dote/TypeJ: 6/I7/97 Grab. <br />DATE/1'IME TEST COMPLETED: 6/22/97 9:30 p.m. <br />Fathead Minnow, 7 days. <br />10 organisms/I50 mL/2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light 8 hours dark. , <br />Measured initially and at 24 hours. <br />Reconstituted water (+I S% hardness of [he receiving <br />water). <br />None was supplied. <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br /> <br />Cee Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />