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W E'. T. Inc. <br />Water & Ertvlranmen(a! TesBnr /nc 479 W'es( SO North. American Fork Utah 84003 (807175ti-8397 <br />• <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: March 10, 1997 <br />• <br />PERMITTEE NAME: Western Fuel Colorado SIGHT ID: New Horizon Mine <br />TEST (AnimaUAgeJ: Fathead Minnow, 6 days. <br />DATE/CIME TEST BEGAN: 3/4/97 7:30 p.m. <br />Fathead Minnow larvae were exposed to diluted etiluent az specified by the permit At the end of the test period Survival was <br />meazured to determine if Acute Toxicity was present in the sample. <br />Auimal Age at Test Start <br />Number of Organisms/Dilutioo Volume/Replicates <br />Food <br />OUTFACE NO: 001 <br />SAMPLE (Date/Type): 3/3/97. <br />DATE/I'IME TEST COMPLETED: 3/8/97 8:15 p.m. <br />Fathead Minnow, 6 days. <br />10 organisms/150 mU2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light 8 hours dark. . <br />Measured initially and at 24 hours. <br />Reconstituted water (+1 S% hardness of the receiving <br />water). <br />None was supplied. <br />Control, 6.25. 12.5, 25, 50, l00%. <br />Aeration <br />Dissolved Orygen <br />Water Replacement <br />Temperature <br />Photo Period <br />PH <br />Dilution Water <br />Receiving Water <br />Sam le Cooceatt <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Since y, <br />Lee Rawlings <br />Water & Env'vonmental Testing, Inc. <br />• Enclosure <br />