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W.E.T. Inc. <br />Water .f Environmental Teslin2lne 479 Wes! SO North. ,4merican Fork Utah 84003 /8011756-8397 <br />• <br />Acute Whole EfflueKt Toxicity Report <br />Ceriodaphnia <br />DATE: March 10, 1997 <br />PERMITTEE NAME: Western Fuel Colorado SICHT ID: New Horizon Mine <br />TEST (AnimaUAge): Ceriodaphnia Q4 hours. <br />DATE/1'IME TEST BEGAN: 3/4/97 7:45 p.m. <br />OUTFACE NO: 007 <br />SAMPLE (Dare/IypeJ: 3/3/97. <br />DATE/1'IME TEST COMPLETED: 3/6/97 8:15 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />DissaWed Orygea <br />Wafer Replacememt <br />• Temperature <br />Photo Period <br />Pb <br />Dilution Water <br />Receiving Water <br />Q4 hours. <br />5 organisms/IS mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. , <br />20 ± ]degrees C. <br />16 hours light S hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (+15% hardness of the receiving <br />water). <br />None was supplied. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincer ' <br />,~~/~~-~ <br />Lee Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />• <br />