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envisioned in the current permit. Transect lengths, quadrat sizes, and other <br />aspects of the design as described in Section 2.1 narrative and depicted in Figure <br />1 of the report differ in various specifics from the methods described on pages 13i <br />and 13ii of the permit. In general the sample layout as described in the report is <br />acceptable for bond release success demonstration sampling, and the systematic <br />design described does have certain advantages over the simple random design. <br />One issue regarding the layout needs to be clarified, with respect to sample size. <br />In a random design, once sample adequacy has been demonstrated, and the <br />relevant minimum sample size achieved, additional sample plots or transects aze <br />not necessary. However, in a systematic design, further sampling is required if <br />sample adequacy/minimum sample size is attained, but sample observations have <br />not been made at each of the pre-selected systematic grid points laid out for the <br />sampled parameter. Further, if sample adequacy has not been achieved upon <br />completion of sampling at each of the initially established grid points, a second <br />systematic grid must be established, with the necessary additional sample points <br />uniformly distributed within the sampled pazcel (under the standazd null <br />framework, where sample adequacy is required). These details do not appear to <br />be cleazly described in the report, and the protocol should be clarified in this <br />regazd. Please modify the protocol to clarify these details in the amended <br />section to be incorporated in the permit. <br />3. Section 2.2 of the report describes the cover sampling method employed. The <br />method employed is generally consistent with the point intercept method <br />described, although the transect length is shorter, and transects were <br />systematically rather than randomly distributed within the sampled pazcels. These <br />differences aze not significant, and the approach described is generally an <br />improvement over that described in the current permit. <br />A potentially more significant departure from the approved plan, is that the <br />approved plan describes a "multiple hit" cover sampling approach, with "hits" on <br />additional vegetation species below the "first hit" to be recorded sepazately. The <br />approved plan indicates that "first hits will be used to calculate total absolute <br />percent cover. "All hits" will be used to calculate relative cover or frequency." <br />This approach is in accordance with the recommended procedure described on <br />page 7 of the Division's October, 1988 Land Use and Vegetation Guideline <br />Document. The method employed in the 2004 sampling, and proposed for bond <br />release sampling in the 2004 monitoring report is a "first hit" point sample <br />method, with no provision for recording of additional species that might occur <br />beneath the plant initially encountered. Given the relatively low cover <br />contribution by woody plant species, it does not appear likely that species <br />composition calculations would be significantly impacted by a "multiple hit" <br />sample approach, as opposed to the "first hit" approach proposed. The cover <br />sample method described is acceptable, but will need to be addressed in the <br />amended sampling section to be incorporated into the permit. <br />