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W.E. ~. Inc. <br />Water & Environmental Testing Lte. 135 !best 400 South, American Fork. Utatt 84003 (8011763-0660 Fax/801)763-0440 <br />v <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: May 31, 2002 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (Anima!/Age): Fathead Minnow, 7 days SAMPLE (Date/Type): 5/23/02 Grab <br />DATE/TIME TEST BEGAN: 5/24/02 11:15 am DATE/T'IME TEST COMPLETED: 5/28/02 12:45 pm <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by EPA Section 9. At the end of the test period <br />Survival was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br />• <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Fathead ILiinnow, 7 days <br />] 0 organisms/I50 mI/2 replicates <br />Fed before; staging test and at 48 hoots, newly, hatched <br />brine shrimp, <br />None required. <br />Measured'daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light 8 hours dazk. <br />Measured initially and at 24 hours. <br />Reconstituted lab water approx. 200 mg/L hardness. <br />None was supplied. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />• <br />Enclosed are data sheets and statistical reports. <br />Sincerely, <br />~\~~G7 i~ <br />ee Rawlings <br />laboratory Director <br />Enclosure <br />Page 2 of 9 <br />