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W.~. T. Inc. <br />Water & Environmental Testin2lnG l35 West 400 South, American Fork, Utah 84003 (8011763-0660 Fax/801)763-0440 <br /> <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: April 2, 20(12 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (Animal/AgeJ: Fathead Minnow, 7 days. SAMPLE (Date/TypeJ: 3/26/2002 Grab. <br />DATElTIME TEST BEGAN: 3/27/2002 1:10 pm DATE/1'IME TEST COMPLETED: 3/31/2002 I:15 pm <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted e8luent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />• <br />Ammal;Age`af Test Start , „ <br />Number of Organisms/Dilution Volume/Replic, <br />Food <br />Aeration <br />Dissolved Oxygen , ~_ . <br />Water Replacement <br />Temperature _ <br />Photo Period <br />Dilution Water <br />Fatheadl4iinnow'Z;days `.°` ~ z :, <br />10 organisms/150 mLl2 replicates <br />Fed before starling test and at 48 hours neivlq ha <br />brine shrimp ,. . <br />None required <br />;Measured dai]y old/neiv. <br />Renewed daily. <br />'20 +i.l .degrees C . - <br />16 hours light 8 hours dark. <br />Measured rnttrally and~at 24 hours :~ ,_a.v -:..; } <br />Reconstituted lab water approx 200 mg/L hardness. <br />SUMMARY <br />Results: Pass Fail <br />There was NO significant effect on survival. <br />Enclosed arc data sheets and statistical reports. <br />Sincerely, ~ ~~ <br />~IU,U 6~.." 7- <br />Rawlings <br />Water Bc Environmental Testing, Inc. <br />I ~ Enclosure <br />Page 2 of 9 <br />