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1' i' . ~. T. Inc. <br /> <br />Water & Environmental Testir:p Inc. 235 West 400 South, American Fork, Utah 84003 (8011763-0660 Fax/80117[i3-0440 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: January 6, 2002 <br />• <br />PERMITTEE NAME: Western Fuels Co. (Acc. Test #I) <br />TEST (AnimaUAge): Ceriodaphnia <24 hours. <br />SITE ID: New Horizon Mine OUTFACE NO: 008 <br />SAMPLE (Da[e/7ypeJ: 1/2/02 Grab. <br />DATE/I'IME TEST BEGAN: I/3/02 2:30 pm <br />DATE/TIME TE5T COMPLETED: 1/5/02 2:40 pm <br />TEST CONDITIONS <br />Ceriodaphnia duliia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period <br />Survival was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Stan; <br />Number ofOrganisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Orygen <br />Water Replacement <br />Temperature <br />Photo Period <br />Q4 hours. <br />5 organisms/15 mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hoots. <br />Reconstituted lab water approx. 200 mg/L hazdness. <br />None was supplied. <br />pH <br />Dilution Water <br />Receiving Water <br />SUMMARY <br />Results: Pass ~~ Fail <br />There WAS significant effect on survival LC50 estimated at 68.3%. <br /> <br />Enclosed are data sheets and statistical reports. <br />Sincerely, ~ ~~_ <br />~lLf!!~ / <br />e Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />