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W.E.T. Inc. <br />Water & Environmental Testing /nc. 235 {Vest 400 SoaUa American Fork, Utah 84003 (8 011 763-0 6 60 FaxB011763-0440 <br />~ Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: January 5, 2002 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 008 <br />TEST (Animal/AgeJ: Ceriodaphnia <2q hours. SAMPLE (Date/TypeJ: 12/26/01 Grab. <br />DATE(FIME TEST BEGAN: 12!27!01 2:35 pm <br />DATE(T[ME TEST COMPLETED: 12/24!01 2:30 pm <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period <br />Survival was measured to de[ennine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br /> <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Q4 hours. <br />5 organisms/15 mU4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted lab water approx. 200 mg/C. hardness. <br />None was supplied. <br />SUMMARY <br />Results: Pass X Fail <br />There WAS significant effect on survival. LCSO estimated at SS.S%. <br /> <br />Enclosed are data sheets and statistical reports. <br />Sincere) , <br />~~~ <br />ee Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />Page 4 of 10 <br />