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.~ <br />Table 1 -Colorado Acute Test Conditions <br />Parameter <br />C. dubia & D. magna <br />Fathead Minnow <br />Test Type <br />Temperature <br />Photoperiod <br />Test Chamber Size <br />Test Sample Volume <br />Renewal Period <br />Age of Organisms <br />Number per Chamber <br />Chambers Per Concentration <br />Feeding Regime Per Chamber <br />Test Duration <br />Stalic Renewal <br />20+/-1°C <br />16 hr light/8 hr dark <br />30 ml <br />20 mI <br />24 hrs <br />< 24 hrs <br />5 <br />4 <br />0.1 ml Algae/YTC <br />48 hrs <br />Static Renewal <br />20 +/-1 ° C <br />16 hr light/8 hr dark <br />600 ml <br />300 ml <br />24 hrs <br />1 to 14 days <br />10 <br />4 <br />0.1 ml Brine Shrimp <br />96 hrs <br />Table 2 -Methods and Equipment <br />Characteristic Method/Equipment EPA Method <br />Alkalinity Titrimetric (APHAe, 1989) EPA 310.1 <br />Ammonia, as Nitrogen Colorimetric Analysis Method EPA 350.1 <br />Chlorine, Total Residual LaMotte STC-CI Colorimeter EPA 330.4 <br />Conductivity Oakton CON 500 Cond. Meter EPA 120.1 <br />Dissolved Oxygen Orion Model 850 DO Meter EPA 360.1 <br />Hardness Titrimetric (APHA, 1989) EPA 130.2 <br />pH Fisher Model 925 pH Meter EPA 150.1 <br />Temperature NIST' calibrated thermometer EPA 170.1 <br />References <br />American Public Health Association. 1992. Standard Methods for the Examination of Water and <br />Wastewater, 18"' Edition. American Public Health Association, Washington, D.C. <br />Colorado Department of Health. 1998. Guidelines for Conducting Whole Effluent Toxicity Tests. Water <br />Quality Control Division, Denver, Colorado 80246-1530. <br />USEPA. 1993. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine <br />Organisms. EPAf600/490/027F, 4~' edition. EMSL, Cincinnati, Ohio 45268. <br />u <br />e American Public Health Association <br />' Natlonal Institute of Standards and Technology <br />