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W.E.T. Inc. <br />Water & Environmenra/ Teslin¢ /nc. 399 West 1200 Nar/h. 8 Orem Ulnh 84057 (801)135-9741 Fax18011135-9560 <br />u <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphrria <br />DATE: September 21, 1998 <br />• <br />l J <br />PERMITTEE NAME: Western Fuels Colorado SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (AnimaUAgeJ: Ceriodaphnia Q4 hours. SAMPLE (Dare/Type): 9/16/98 Grab. <br />DATEII'IME TEST BEGAN: 9/17/98 12:00 p.m. DATE/TIME TEST COMPLETED: 9/19/98 2:30 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of [he test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Orgaoisms/Dilutioo Volume/Replicates <br />Faod <br />Aeration <br />Dissolved Orygen <br />Water Replacement <br />Temperature <br />Photo Period <br />SUMMARY <br />pH <br />Dilution Water <br />Receiving Water <br />Sam le Coeceuti <br />Q4 hours. <br />5 organisms/IS mU4 replicates. <br />Fed YTC and Algae before starting test. <br />None requved. <br />Measured daily old/new. <br />Renewed daily. <br />20 + 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water LI S% hardness of the receiving <br />water). <br />None was supplied. <br />Results: X Pass Fail <br />There was NO significant effect an survival. <br />Enclosed are data sheets and statistical reports. <br />Sincere) <br />u GZG U ~" <br />Lee Rawlings / <br />Water & Environmental Testing, Inc. <br />Enclosure <br />