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W ~'. T. Inc. <br />r & Environmentol Tesrinr /nc. 179 Wesr SO Narrh, American Fork Ulah 81003 /801)756-8397 <br />, <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnin <br />DATE: March 23, 1998 <br />PERMITTEE NAME: Western Fuel Colorado SIGHT ID: New Horizon Mine <br />TEST (AnimaUAge): Ceriodaphnia <24 hours. <br />DATEl1'-ME TEST BEGAN: 3/17/98 2:00 <br />TEST CONDITIONS <br />OUTFACE NO: 001 <br />SAMPLE (Dale/Type): 3/16/98 Grab. <br />DATE/I'IME TEST COMPLETED: 3/19/98 2:00 p.m.. <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by [he perm it. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />NumberotOrganisms/Dilution Volume/Replicates <br />Food <br />• <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Pho[o Period <br />pH <br />Q4 hours. <br />5 organisms/IS mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water LIS%hardness of the receiving <br />water). <br />None was supplied. <br />Dilution Water <br />Receiving Water <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincere~l(, <br />•~/ V/u "L L <br />Lee Rawlings <br />Water & Environmental Testing, Inc. <br />• Enclosure <br />