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W.~'. T. Inc. <br />Water & Environmental Testing lnc. 479 West SO Nonh, American Fork Utah 84003 (80!)756-8397 <br />• <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: December 23, 1997 <br />• <br />PERMITTEE NAME: Western Fuels Colorado SIGHT ID: New Horizon Mine <br />TEST (Animal/Age): Fathead Minnow, 7 days. <br />OUTFACE NO: 007 <br />SAMPLE (Da(e/Type) 12/9/97 Grab. <br />DATE/I'IME TEST BEGAN: 12/10/97 3:45 p.m. DATElI'IME TEST COMPLETED: 12/14/97 4:15 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted eRluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Auimal Age at Test Start <br />Number of Organisms/Dilutioo Volume/Replicates <br />Food <br />Fathead Minnow, 7 days. <br />10 organisms/I50 mL/2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + 1 degrees C. <br />I6 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (±I S% hardness of the receiving <br />water). <br />None was supplied. <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Sam le Concentt <br />SUMMARY <br />100%. <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Smcerel / <br />,~.L; ~~Z <br />Lee Rawlings <br />Wa[er & Environmental Testing, Inc. <br />~~s~~~nn~ r~~i <br />~1IJJffr_~ -~-~II ~~ i <br />~:~i~; ,11 DEC 2 9 1997 <br />Enclosure <br />