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W ~'. T. Inc. <br />-ter & Environmental Testine Inc. 479 West 50 Nonh. American Fork Utah 84003 (80/1756-8397 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: March 23, 1998 <br />PERMITTEE NAME: Western Fuel Colorado SIGIfT ID: New Horizon Mine <br />TEST (.4nimul/AgeJ: Ceriodaphnia <14 hours. <br />OUTFACE NO: 007 <br />SAMPLE (Dale/Type): 3/16/98 Crrab. <br />DATE/1'IME TEST BEGAN: 3/17/98 I :45 p.m. DATE/I'IME TEST COMPLETED: 3/19/98 2:30 p.m.. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilu[ion Volume/Replicates <br />Food <br /> <br />Aeration <br />Dissolved Oxygeu <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Q4 hours. <br />5 organisms/15 mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + I degrees C. <br />I6 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (±FS°~6 hardness of the receiving <br />water). <br />None was supplied. <br />Dilu[ioo Water <br />Receiving Water <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerel <br />acv !~,B <br />ee Rawlings <br />Water & Environmental Testing, Inc. <br />• Enclosure <br />