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W.E.T. Inc. <br />Water & Environmental Testing /nc. 479 Wes! 50 North. American Fork Ulah 84003 (8 011 75 6-83 9 7 <br />• Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: March 23, 1998 <br />r1 <br />PERMITTEE NAME: Western Fuels Colorado SIGHT 1D: New Horizon Mine <br />TEST (.9nimaUAge): Fathead Minnow, 7 days. <br />OUTFACE NO: 007 <br />SAMPLE (Date/Type) 3/16/98 Grab. <br />DATE/1'IME TEST BEGAN: 3/17/98 2:45 p.m. DATE/TIME TEST COMPLETED: 3/21/98 3:35 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of OrganismslDilutioo Volume/Replicates <br />Food <br />Fathead Minnow, 7 days. <br />10 organisms/I50 mU2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily o!d/new. <br />Renewed daily. <br />20 + I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (±IS°i° hardness of the receiving <br />water). <br />None was supplied. <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincere , <br />~. G i Lv , <br />Lee Rawlings <br />Water & Environmental Testing, Inc. <br />• Enclosure <br />pH <br />Dilution Water <br />Receiving Water <br />Sam le Coocent~ <br />