PERMFILE131137

• Permit No: C1980005
• IBM Index Class Name: Permit File
• Doc Date: 12/11/2001
• Doc Name: LABORATORY PROCEDURES
• Section_Exhibit Name: TAB 06 APPENDIX 6-2
• Media Type: D
• Archive: No

• Available Phosphorus in Mine Soils
(Modified method of Olsen, et al, 1954)
Solutions:
1. Sodium bicarbonate (NaHCO ) solution, O.SM_, adjusted to pH
8.5 with 1 M NaOH. Mineral oil added to avoid exposure to
the air; stored in a polyethylene container and made fresh
every 2 months.
2. Ammonium molybdate, (NH ) Mo O 4H O, solution:
Dissolve 15 g. in 300 m~ ~f warm4disti~led and deionized
water. Filter if cloudy and allow to cool. Gradually
add 342 ml of conc. HCL and mix. Dilute to 1 liter.
3. Concentrated SnCl2 2H2O solution: 10 g. of large crystals
dissolved in 25 mI. conc. HC1. Store in a brown glass
bottle in a refrigerator. Prepare fresh every 2 months.
a) Dilute SnCl add 0.5 ml of the conc. SnCl solution
to 66 ml. distilled and deionized water. 2Prepare
the dilute solution for each set of determinations.
• 4. Standard P solution: Weigh 0.4393 g. KH2PO4 into a 1 liter
vclumetric flask. Add 500 mis. distilled and deionized
water and dissolve the salt. Dilute to 1 liter, and add
5 drops of toluene to reduce microbial growth.
5. Dilute P solution: Dilute 20 ml. (pipet of the P solution
from 14) to 1 liter with distilled and deionized water.
This solution contains two micrograms of P per ml.
Procedure:
1. Add 1.OOg of
Na HCO solu
the fl~sk.
2. Shake for 30
on a Burrell
60 mesh rock or soil sample, and 20 ml of
lion to a 50 ml Erlenmeyer flask. Stopper
min., at 20oC, using a shaking speed of 2
Wrist Action shaker.
3. Centrifuge for 15 minutes at 2,000 R.P.M.
4. Pipet 10 ml of supernate into a 25 ml volumetric flask and
dilute to volume. Transfer to a test tube.
5. Slowly add 5 ml of Ammonium Molybdate solution. Mix well.
• 6. Pipet 1 ml of dilute SnC12 solution into filtering flask,
and mix.
7. Read absorbance on a spectrophotometer a 660 mu, after
10-20 min.
6-62