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• Determination of Cu, Cd, Mn, Ni, Zn - Continued <br />d. Centrifuge and pass through a 0.45 micron filter, retaining <br />filtrate in a stoppered test tube for analysis. <br />e. Analyze the filtrate for Cu, Fe, Zn,. Pb, Cd, Ni and Mn <br />by atomic absorption spectrophotometry. If dilutions <br />are necessary, they should be made with DTPA-TEA solution. <br />Calculations <br />ppm in solution x 20 ml = ppm in soil <br />10 g <br />ppm = ug/ml (W/V) or ug/g (W/W) <br />Above calculation determined as follows: <br />x ppm in solution = x ug/ml <br />x ug/ml x 20m1 = total ug extracted by DTPA-TEA <br />• iQx ug = Xug/g in soil <br />4 <br />• <br />6-52 <br />