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PERMFILE130584
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PERMFILE130584
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Last modified
8/24/2016 10:31:30 PM
Creation date
11/25/2007 10:32:12 PM
Metadata
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Template:
DRMS Permit Index
Permit No
C1981016
IBM Index Class Name
Permit File
Doc Date
12/11/2001
Doc Name
Aquatic Resources
Section_Exhibit Name
APPENDIX D CONTINUED
Media Type
D
Archive
No
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beak <br />aliquots to determine (1) generic composition of the periphyton, (2) dry <br />• and ash-free weight, and (3) chlorophyll a content. <br />The generic composition of the periphyton was determined by filtering <br />a suitable aliquot onto a 0.45um pore-size membrane fil*_er, making the <br />filter transparent, then identifying to genus and counting 200 discrete <br />algae particles at a magnification of 400x. Taxonomic identifications were <br />made using the descriptions of: Patrick and Reimer 1966, 1975; Drouet 1959; <br />Geitler 1932; Bourrelly 1968; and Prescott 1962, 1970. <br />The procedure for determining the dry weight and dry weight organic <br />matter (ash-free weight) of the periphyton entailed removing visible insect <br />larvae from the periphyton, filtering the entire quantity of remaining <br />material onto tared GF/C glass fiber filters, drying the filters at 105°C, <br />weighing the filters, then ashing the filters at SOOoC and re-weighing <br />• (APHA 1976). <br />The procedure for determining chlorophyll a, another measure of biomass, <br />entailed filtering periphyton material onto 0.45um pore-size membrane filters, <br />grinding the filters to disrupt algae cells, then recording absorbances on <br />a spectrophotometer with a resolution of Bum (APHA 1976). <br />4.2 Results and Discussion <br />Attached algae on the stream bottom substrate of the tlorth Fork Gunnison <br />River was sampled at four stations, C, D, E, and F. Generic analysis of the <br />periphyton showed diatoms of the division Chryspophyta were the most numerous <br />genera; blue-green and green algae, of the divisions Cyanophyta and Lhlorophyta, <br />respectively, were represented by only three genera (Table 4.0-1). The most <br />• abundant genus at stations C, D, ad E was the blue-green Schizothrix (Tables <br />4.0-2, 4.0-3, and 4.0-4) and at station F the diatom genus CYmbella (Table 4.0-5). <br />4-2 <br />
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