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• -Native Perennial Grasses -Native Subshrubs <br />- Introduced Perennial Graminoids -Native Trees <br />-Native Shrubs -Succulents <br />- Lichens -Bryophytes (mosses) <br />-Fungi -Algae <br />Cover sampling methods set forth above will be used in each BRB and each reference azea during the <br />2005 and 2006 scheduled sampling event. <br />PRODUCTION SAMPLING <br />Production samples will be taken at initially selected sample points as shown in the table above. Thu is the minimum <br />number of sampler for ure in a `reverse null hypothesir"test (CDMG 1003, proposed rules changes, 4.15.11(1J(c)). <br />If the production values from the reclaimed areas angnater than the reference area means, the hypothesis of reclamatiox <br />adequacy forproductsax will be terted using the `Severre xulllypotheris"(.ra Hypothuar Testixg, be%w). <br />Biomass production will be assessed within one (1.0) squaze meter plots located at the randomly <br />placed sample points. Sampling will occur between July 15 and August 31, 2005, and in the same <br />period in 2006. Cattle grazing on BRB and reference area sites will be deferred until after the clip <br />sampling is complete. At each sample plot, a circuiaz plot (hoop) enclosing one (1.0) squaze meter <br />will be placed on the ground and anchored. Herbaceous plant species present will be clipped as <br />closely to the ground as possible and placed separately into labeled paper bags. Clipped plants will be <br />grouped into the following life forms and bagged: <br />• -Native Annual f Biennial Forbs -Introduced Atmual /Biennial Forbs <br />- Native Annual Grasses -Introduced Atmual Grasses <br />-Native Perennial Forbs -Introduced Perennial Forbs <br />-Native Perennial Grasses & Grasslikes -Introduced Perennial Gratninoids <br />Trees, full shrubs, subshrubs, cacti, lichens, mosses, algae, and agavoid subshrubs (Yucca spp.) will <br />no[ be clipped. Clipped materials will be oven-dried at 105° C and weighed to the nearest 0.1 gm. <br />COMPILATION OF A PLANT SPECIES LIST <br />Cumulative plant species lists for each of the BRB areas and the reference areas will be separately <br />compiled from observations during 2005 and 2006 sampling work. <br />SPECIES DIVERSITY (DENSITY) <br />Species presence will be noted within the azea one meter to either side of the transect (50 m x 2 m = <br />100 m~. These presence data along with point-intercept data will be used to produce values for <br />species density expressed on a per 100 mz basis. <br />ASSESSMENT OF RECLAMATION ADEQUACY <br />General <br />Formal (statistical) hypothesis testing (see below) will be undertaken for BRB-3, BRB-4 and BRB-5 <br />(post-law, Permanent Program lands). For pre-law lands mined prior to August 3, 1977 (BRB-1), <br />• qualitative information and quantitative data collected from sampling will be used in a discussion of <br />the completion of reclamation with "all reasonable diligence." For lands mined and reclaimed <br />ESC0.9ssoaates 13-13-5 TRIO *Ol/07 <br />