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2. Whole Effluent Toziciri Testing Requirements -Acute WET Testing-Outfall 001 (b) Failure of Test and Divisionl~otification An acute WET test is failed «'henever the LCS°, which represents an estimate of the effluent concentration which is let 50% of the test organistns in the time period prescribed by the test, is found to be less than or equal to (00% effluent. • permittee must provide written notification of the failure of a WET test to the Division, along with a statement as to orl the Preliminary Toxicity Incident ("PTY")R'oxicity Identification Evaluation ("TIE") investigation or accelerated test being performed (see next section). 1`fotification must be received by the Division within 14 calendar days c demonstration of acute WET in the routine required test. "Demonstration" for [he purposes of Parts I.A.3.(b),(c),(t (f) means no later than the last day of the laboratory test. (c) Automatic Compliance Schedule IJDOn Failure of Test If a routine acute WET test is failed, the following automatic compliance schedule shall apply. As part of this, the pen shall either. (i) Proceed to conduct the PTLTIE investigation as described in Part LA.3.(d), or (ii) Conduct accelerated testing using the single species found to be more sensitive. If accelerated testing is being performed, the permittee shall provide written notification of the results With calendar days of completion of the "Pattern of Toxicity"/"iIo Tonicity" demonstration. Testing will be at lease every two weeks for up to fire tests until 1) hvo consecutive tests fail or three of five tests fail, in which case a patt toxicity has been demonstrated or, 2) two consecutive tests pass or three of five tests pass, in which case no pattem of tc has been found. If no pattern of toxicity is found, the toxicity episode is considered to be ended and routine testins resume. If a pattem of toxicity is found, a PTVTIE investigation is to be performed. If a pattem of toxicity demonstrated but a significant level of erratic toxicity is found, the Division may require an increased frequency qt't monitoring or some ocher modi£ed approach. (d) PTUTIE • The results of the PTIITIE investigation are to be received by the Division within 120 dav5 of the demonstrat acute WET in the routine test, as defined above, or if accelerated testing is performed the date the pattern of toxi demonstrated. A status report is to be Drovided to the Division at the 30 60 and 90 day points of the PT investigation. The Division may exteod the period for investigation where reasonable justification exists. A request extension must be made in writing and received before the 120-day deadline. Such request must include a justificadc supporting data for such an extension. The permittee may use the time for investigation to conduct a PTI or move directly into the TIE. A PTI consists of a search for possible sources of WET, which might reveal causes of such toxicity and appropriate conective actions mo;e and cost effectively than a formal TIE. If the PTI allows resa'.ution of the W'ET incident, the TIE need not necrsat conducted. If, however, WET is not identified or resolved during the PTI, the TIE must be conducted within the allo~ve~ day period. Any permittee that is required to conduct a PTUTIE investigation shall do so in conformance with procedures identified following documents, or as subsequently updated: 1) Methods for Aquatic Toxicity Identification Evaluations P Toxicity Characterization Procedures, EPA/600/6-91/003 Feb. 91 and 2) Methods for Aquatic Toxicity Identifi Evaluations Phase II Toxicity Identification Procedures. EPA/600(3-88103 S Feb. 1989. A third documeat in this series is Methods for Aquatic Toxicity Identification Evaluations Phase III Toxicity Confu-t Procedures, EPA/600/3-88!036 Feb. 1989. As indicated by the title, this procedure is intended to confirm that the sus toxicant is truly the toxicant. This investigation is optional. Within 90 days of the determination of the toxicant or no later than 210 days after demonstration of toxicity, w sooner, a control program is to be developed and received by the Division. The program shall set down a meutc procedure for elimination of the toxicity to acceptable levels.