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SPIRANTHES DILUV/ALfSSORVEY REPORT <br />Methods <br />Prior to beginning the field survey for Spiranthes diluvialis, the project area was <br />reviewed to determine the location and extent of any potential habitat for Spiranthes <br />diluvialis. The following types of habitat were considered to potentially support <br />populations of Spiranthes diluvialis- <br />1. Areas determined to be jurisdictional wetlands; <br />2. Seasonally moist azeas neaz springs, lakes, irrigation ditches, or perennial streams <br />and their associated flood plains; <br />3. Old stream channels and alluvial terraces; <br />4. Subirrigated meadows; <br />5. Areas supporting vegetation indicative of seasonally wet areas or areas dominated <br />by vegetation considered to be facultative wet. <br />Additionally, NWI maps and SCS soils maps were reviewed for areas mapped as <br />wetlands or hydric soils or soils with hydric inclusions: It was the intent of the survey to <br />be overly inclusive in detetinining azeas to be surveyed for the presence or absence of <br />Spiranthes diluvialis. Before the search, ERO biologists visited the Spiranthes diluvialis <br />reference azea in Wheat Ridge, Colorado to observe and photograph the plant in bloom. <br />Each area considered to be potential habitat for Spiranthes diluvialis was carefully <br />surveyed by walking a pattern of parallel transects through the potential habitat. The <br />interval between transects was determined sepazately for each site depending on the <br />height of vegetation and the distance that the surveyor was confident in determining the <br />radius of accurate observation. Typically, the surveyor slowly walks 3 to 5 paces, stops, <br />and slowly rotates 360° while continually viewing the vegetation within the <br />predetermined radius or belt of vision, while looking for the presence of Spiranthes <br />diluvialis. The surveyor is very familiaz with both the vegetative chazacteristics as well <br />as the inflorescence of Spiranthes diluvialis. The surveyor did not solely key on <br />inflorescence and color. Additionally, two infrequently occumng species (Lobelia <br />siphilitica and Limnorchis hyberborea) known to occur with Spiranthes diluvialis were <br />looked for as part of the survey. <br />ERO <br />Resourees <br />Corporation <br />